myd88  (Bio-Rad)

Journal: Journal of Nanobiotechnology

Article Title: Oral administration of probiotic spores-based biohybrid system for efficient attenuation of Salmonella Typhimurium-induced colitis

doi: 10.1186/s12951-025-03468-x

Figure Lengend Snippet: The anti-inflammatory mechanism of BCs@PME-Au in S . Tm induced colitis. ( A ) The specific schematic diagram of BCs@PME-Au exerting anti-inflammatory. ( B ) The representative western blot images of TLR4, MyD88 and NF-κB p65. ( C ) The quantitative expression analysis of TLR4, NF-κB p65 and MyD88. ( D ) The representative immunofluorescence images and quantitative analysis of TLR4, NF-κB p65 and MyD88. ( E ) The expression changes of inflammatory cytokines of mice with different treatments. Data were presented as mean ± SD. Statistical analysis by one-way ANOVA with Tukey’s multiple-comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001, n.s, no significance

Article Snippet: After blocking with 5% non-fat milk for 1 h, the membranes were probed with primary antibodies (TLR4 (D8L5W) Rabbit mAb, 14358, Cell Signaling Technology; MyD88 (D80F5) Rabbit mAb, 4283, Cell Signaling Technology; NF-κB p65 (D14E12) Rabbit mAb, 8242, Cell Signaling Technology; β-Actin (8H10D10) Mouse mAb, 3700, Cell Signaling Technology) overnight at 4 °C and then incubated with the secondary antibodies (Anti-Rabbit IgG, HRP-linked, 7074, Cell Signaling Technology).

Techniques: Western Blot, Expressing, Immunofluorescence